Effects of various pH in testing bacterial endotoxins / 中国药学杂志
Chinese Pharmaceutical Journal
;
(24): 163-1998.
Article
Dans Chinois
| WPRIM
| ID: wpr-598264
ABSTRACT
OBJECTIVE:
To study the effect of samples having different pH values in the bacterial endotoxins test (BET).METHODS:
The BET was conducted as an assay of the concentration of endotoxins by using kinetic-turbidimetric technique. Samples having different pH values but containing same concentration reference standard endotoxin (RSE) were tested by TAL/LAL reagents. The average result on the standard curve was calculated and the mean recovery observed.RESULTS:
The reagents had three highly sensitivities in pH 5.20, pH 7.83/pH 6.23 and pH 10.55. After adjustment to pH 6 to 8, the mean recovery was 76.5%~115.9% or 85.8%~99.8%. When the sample pH value was less than 3 or more than 12, the test was unsuitable for the inhibition test, and the mean recovery was less than 56.8%.CONCLUSION:
It is necessary to adjust the pH of the solution to be tested in the BET, adjust the sample to pH (6.5~7.5) when using TAL reagents, and adjust to pH (6.2±0.1) or pH(6.7~7.8) when using LAL reagents.
Texte intégral:
Disponible
Indice:
WPRIM (Pacifique occidental)
langue:
Chinois
Texte intégral:
Chinese Pharmaceutical Journal
Année:
1998
Type:
Article
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