Screening of human phage single chain antibody against the recombinant surface protein of Streptococcus mutans and identification of its biological activities / 中国人兽共患病学报
Chinese Journal of Zoonoses
;
(12): 201-204,208, 2010.
Article
Dans Chinois
| WPRIM
| ID: wpr-598287
ABSTRACT
To isolate human phage single chain antibody against surface protein of Streptococcus mutans,the recombinant surface protein of S.mutans(rAP) was used to coat the immune tubes and the phage single chain antibody was prepared through pDAN5 phage antibody library after 5 rounds of panning.The eluted phage was enriched nearly 30 times.In these ways,13 positive clones were obtained and found to be able to bind with rAP in ELISA assay.Then one of the 13 positive clone phage plasmid was used to infect E.coli HB2151 to induce the expression of the non-fusion single chain antibody (ScFv) with IPTG induction.As demonstrated by SDS-PAGE,the molecular mass of this single chain antibody was proved to be 30 kDa and the amount of expression constituted to 30% of the total bacterial proteins.Apparently,the human phage single chain antibody against surface protein of S.mutans with biological activity was successfully screened.
Texte intégral:
Disponible
Indice:
WPRIM (Pacifique occidental)
Type d'étude:
Etude diagnostique
/
Étude pronostique
/
Étude de dépistage
langue:
Chinois
Texte intégral:
Chinese Journal of Zoonoses
Année:
2010
Type:
Article
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