Separation and identification of quercetin,genistein and their sulphate derivatives with micellar paper chromatography / 中国药学杂志

ABSTRACT

OBJECTIVE：To investigate the use of micellar paper chromatography on the isolation and identification of flavonoids and their soluble derivatives.METHOD:Quercetin,Genistein and their sulphate derivatives were separated and identified by SDS solution above critical micelle concentration (CMC) on paper chromatography.The concentration of SDS,ratio of organic modifier and their concentration,fluorescence were investigated.RESULTS:The best concentration of SDS micelle was 0.01～0.02mol*L-1,and the spot effects of micellar chromatography could be improved by adding 2%～4% isopropanol (or n-amyl alcohol)into the mobile phase.CONCLUSION:The simple,rapid,accurate and qualitative method could be used to analyze and isolate flavonoids and their soluble derivatives.