Effect of Danhong injection on respiratory inhibition induced by hypoxia / 中国病理生理杂志

ABSTRACT

AIM: To investigate the effect of Danhong injection on respiratory inhibition induced by hypoxia and its related mechanism .METHODS:The electromyogram of the diaphragm was monitored to observe the respiratory re-sponses of the rats to hypoxia .The expression of acid-sensingion channel 1a (ASIC1a) in the brainstem after hypoxia was detected by the technique of immunohistochemical staining .RESULTS: The respiratory responses of the rats to hypoxia were initiatory excitation and consequent inhibition .In contrast to pre-hypoxia, the respiration of the rats in hypoxia group was inhibited 30 min after hypoxia (P<0.05), but the respiration of the rats in hypoxia plus Danhong group was still ex-cited (P<0.05), indicating that the respiration of the rats in hypoxia plus Danhong group was not yet depressed at the same time.The ASIC1a positive neurons were detected in the nuclei of trapezoid body and solitary tract .Compared with control group, the expression of ASIC1a was obviously enhanced after hypoxia .In contrast to hypoxia group , the expression of ASIC1a in the rats in hypoxia plus Danhong group was remarkably reduced (P<0.05).CONCLUSION:Danhong in-jection resists the occurrence of respiratory inhibition after hypoxia , and ASIC1a may participate in this process .