Detection of ESBLs-producing Escherichia coli and Klebsiella pneumoniae in children with recurrent upper respiratory tract infections / 国际检验医学杂志

ABSTRACT

Objective To detect the extended-spectrumβ-lactamases(ESBLs)producing pathogens by the throat swab culture in children with recurrent upper respiratory tract infections,and to analyze the drug resistance.Methods The throat swab culture, bacterial strain identification and drug sensitivity test were performed in the children with recurrent upper respiratory tract infec-tions by using the American MICROSCAN bacterial analysis software.The results were interpreted according to the standards of American Clinical and Laboratory Standards Institute(CLSI).The retrospective analysis was performed.Results In 260 strains of isolated Gram-negative bacteria,186 cases were Escherichia coli and 74 cases were Klebsiella pneumoniae,in which ESBLs-produ-cing strains were 78 cases and 42 cases respectively.The detection rate of the ESBLs-producing strains in Escherichia coli was 43.01%(80/186),and which in Klebsiella pneumoniae was 48.65%(36/74).The resistance rates of ESBLs-producing strains to commonly used antibiotics were significantly higher than those of non-ESBLs-producing strains,both strains were highly sensitive to imipenem,piperacillin sulbactam and amikacin,but showed the multiple drug resistance to some cephalosporin antibiotics.Conclu-sion The detection rate of ESBLs-producing pathogens in the children with recurrent upper respiratory tract infection is higher. These bacteria show the higher resistance to conventional antibiotics,and it is the key to ensure clinical antimicrobial therapeutic ef-ficacy according to the effective pathogen culture and the drug susceptibility testing.