Expression profile of microRNAs under different lever of HER-2 in human breast cancer MCF-7 cells / 临床与实验病理学杂志

ABSTRACT

Purpose To screen out miRNA expression profile related to HER-2 via observing the differential expression of miRNA ex-pression of human breast cancer cells MCF-7 and MCF-7/HER-2. Methods After cultivating human breast cancer cells MCF-7 and MCF-7/HER-2, the expression profile of miRNAs was detected by miRNA microarrays. Results Compared to MCF-7 cells, two hundreds and seventeen HER-2 related miRNAs were identified from breast cancer MCF-7/HER-2 cells, including 123 up-regulated and 94 down-regulated miRNAs. Of significant differentialy expressed miRNAs, hsa-miR-141 and hsa-miR-1299’s target genes have multiple biological functions. Conclusion The differentially expressed miRNAs under different lever of HER-2 in human breast cancer MCF-7 cells were obtained, which may provide a basis for studying HER-2 functions and roles in breast cancer.