Th17 Responses Are Not Induced in Dextran Sodium Sulfate Model of Acute Colitis
Immune Network
;
: 416-419, 2011.
Article
Dans Anglais
| WPRIM
| ID: wpr-60130
ABSTRACT
Dextran sodium sulfate (DSS) is a widely used chemical model for inflammatory bowel disease (IBD). It is thought that imbalances in the T helper (Th) cell subsets contribute to IBD. Recent studies suggest that the acute DSS-colitis model is polarized toward a Th1/Th17 profile based on RT-PCR analysis of colonic tissues. In the current study we determined whether colonic Th cells from DSS-colitis mice were skewed toward the Th17 profile. Mice were treated with 5% DSS for 7 days and colonic T cells isolated and examined for production of IFN-gamma (Th1 cell), IL-4 (Th2 cell) and IL-17 (Th17 cell) by intracellular flow cytometry. We found that the percentage of colonic Th17 cells were similar to non-treated controls but the percentage of Th1 cells were elevated in DSS-colitis mice. These results suggest that in the acute DSS-colitis model the colonic Th cells exhibit a Th1 profile and not a Th17 profile.
Texte intégral:
Disponible
Indice:
WPRIM (Pacifique occidental)
Sujet Principal:
Sodium
/
Sulfates
/
Lymphocytes T
/
Maladies inflammatoires intestinales
/
Dextrane
/
Interleukine-4
/
Colite
/
Côlon
/
Lymphocytes auxiliaires Th1
/
Interleukine-17
Type d'étude:
Étude pronostique
Limites du sujet:
Animaux
langue:
Anglais
Texte intégral:
Immune Network
Année:
2011
Type:
Article
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