Determination of Coenzyme A in Coenzyme Complex for Injection by HPLC / 中国药师
China Pharmacist
;
(12): 390-391,392, 2016.
Article
Dans Chinois
| WPRIM
| ID: wpr-603455
ABSTRACT
Objective:
To establish an HPLC method for the determination of coenzyme A in coenzyme complex for injection.Methods:
The content determination was performed on an Intersil ODS-3 column with methanol-pH 6. 5 phosphate buffer solution (10∶90) as the mobile phase. The detection wavelength was 259 nm and the flow rate was 1. 0 ml·min-1 . The column temperature was 30℃ and the injection volume was 20 μl.Results:
The linear range of coenzyme A was 1.624-32.482 u·ml-1(r=0.999 9). The average recovery was 102. 36% and RSD was 1. 14%(n=6).Conclusion:
The method is simple, accurate and reproducible, and it can be used for the quality control of coenzyme complex for injection.
Texte intégral:
Disponible
Indice:
WPRIM (Pacifique occidental)
langue:
Chinois
Texte intégral:
China Pharmacist
Année:
2016
Type:
Article
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