Establishment of Method and Verification for Leukocyte Differential Count of Sysmex Hematology Analyzer / 现代检验医学杂志

ABSTRACT

Objective To calibrate the leukocyte differential counts and compare between all sysmex hematology analyzers, making the results accurate and reliable.Methods Used the manual visual microscope method as a reference method for leu-kocyte differential count to detect the fresh blood and then did assignment.The target value,calibrating the leukocyte differ-ential counts of Sysmex XE-2100,XS-800i and XN-20 (A1)with the manual value fresh blood,was calibrated and then com-pared hematology analyzers with another fresh blood.Results The leukocyte differential counts of all hematology analyzers were within the 95% confidence interval of the manual visual microscope method.Comparing with the reference system,the correlation coefficient(r)of NEUT%,LYM%,MON%,EOS% and BASO% of XS-800i analyzer were 0.99,0.99,0.98, 0.98 and 0.59,respectively.The correlation coefficient (r)of NEUT%,LYM%,MON%,EOS% and BASO% of XN-20 (A1)analyzer were 0.99,0.99,0.97,0.98 and 0.55,respectively.XS-800i and XN-20 (A1)NEUT%,LYM%,MON% and EOS% were within confidence interval 100%.XS-800i BASO% were within confidence interval 95%.XN-20 (A1)BASO%were within confidence interval 90%.Conclusion This scheme could meet the WS/T 246-2005 demands.In order to ensure the accuracy and the results of mutual recognition of the leukocyte differential counts,the comparison analysis of the results of the manual visual microscope method and hematology analyzer were made.