Determination of methomyl in human blood by LC/MS / 中国法医学杂志
Chinese Journal of Forensic Medicine
; (6): 367-368,372, 2016.
Article
de Zh
| WPRIM
| ID: wpr-604671
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WPRO
ABSTRACT
Objective To develop a sensitive and simple LC/MS method for determination of methomyl in blood samples.Methods Methomyl was extracted from blood by liquid-liquid extraction with ethyl acetate, and then analyzed on a Zorbax SB-C18 (2.1mm×50mm, 5μm) column. The mobile phase consisted of acetonitrile-0.1% formic acid with gradient elution, at a lfow rate of 0.5 mL/min, at 40℃. LC-MS was performed in ESI source with MRM mode for quantiifcation.ResultsThe linear range of the concentration were 0.05~2.0μg/mL for methomyl (r>0.995). The relative recoveries of methomyl were in the range of 90%~108%. The RSDs of intra-days and inter-day were both less than 15%.Conclusion The method is a simple, quick, sensitive and could be used for determination of methomyl in blood samples.
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Indice:
WPRIM
langue:
Zh
Texte intégral:
Chinese Journal of Forensic Medicine
Année:
2016
Type:
Article