Expression of CaMKⅡδ in Cerebral Cortex Following Traumatic Brain Injury / 法医学杂志

ABSTRACT

Objective To observe the time-course expression of calcium-calmodulin dependent protein ki-naseⅡδ (CaMKⅡδ) in cerebral cortex after traumatic brain injury (TBI). Methods The TBI rat model was established. The expression of CaMKⅡδ in cerebral cortex around injured area was tested by Western blotting and immunohistochemical staining . Results Western blotting revealed expression of CaMKⅡδ in normal rat brain cortex. It gradually increased after TBI, peaked after 3 days, and then returned to normal level. The result of immunohistochemical staining was consistent with that of West-ern blotting. Conclusion The expression of CaMKⅡδ around injured area after TBI increased initially and then decreased. It could be used as a new indicator for wound age determination following TBI.