Analysis on drug resistance rate and homology of Pseudomonas aeruginosa by ICU nosocomial infection monitoring / 国际检验医学杂志

ABSTRACT

Objective To investigate the drug resistance rate and homology of Pseudomonas aeruginosa isolated from ICU in Mudanjiang Municipal First People′s Hospital from January to June 2015 to understand its prevalence situation in ICU and provide a basis for the rational prevention and control of nosocomial infection.Methods The Vitek-2 Compact fully automatic microbiologi-cal identification instrument was adopted to perform the drug resistance analysis on 126 strains of Pseudomonas aeruginosa from ICU detected by different pathways in the first half of 2015.The homology of bacterial strains was analyzed by pulse-field gel elec-trophoresis(PFGE).Results Two nosocomial infection monitorings were performed in ICU from January to June 2015.Four strains of Pseudomonas aeruginosa isolated from the nurse hand,medicative cart and doorknob of ICU ward all were sensitive strains;122 strains of Pseudomonas aeruginosa were detected in 110 ICU inpatients,in which 38 multi-drug resistant strains were detected from 18 ICU inpatients.The homology analysis was performed in 38 multi-drug resistant strains and 4 strains detected by nosocomial infection monitoring,these strains included 5 groups (A-E),which was dominated by the clone types of A,B and C, while the strains detected by nosocomial infection monitoring all were clone type B.Multiple subtype infection was detected in 4 pa-tients.Conclusion The infection situation of Pseudomonas aeruginosa is serious in ICU,no epidemic outbreak of strains detected by nosocomial infection monitoring exists.The cross prevalence of multiple clone strains exists in inpatients.