Analysis of mycoplasma pneumoniae nucleic acid testing positive cases of children in Guangzhou / 国际检验医学杂志

ABSTRACT

Objective To analysis the clinical features of mycoplasma pneumoniae nucleic acid(MP-DNA)testing positive cases of from the region's children,provide the basis for clinical diagnosis and treatment.Methods Outpatient and hospitalized patients with respiratory infection symptoms were sampled pharyngeal swab in the region from 2014 to 2015.After DNA was extracted,polymerase chain reaction method was conducted to detect the MP gene.At the same time,blood routine and mycoplasma pneumoniae antibody were detected from drawing blood.Results There were 209 cases of MP-DNA positive from 2018 cases in children with respiratory tract infection,the positive rate was 10.36 ％.MP-DNA positive rate in 3-＜ 14 years old age group was 18.25 ％,which was higher than other age groups(P＜0.05).The positive rate was 16.11％ from July to September,which was higher than in other month groups(P＜0.05).The positive rate of girl (13.04 ％) was significantly higher than that of boy (8.79 ％) (P＜ 0.05).WBC count was higher than normal for 22.01 ％,normal accounted for 49.28 ％,lower than normal for 28.71％ in MP-DNA positive children.Serum MP-IgG positive rate was 69.86％ in MP-DNA positive children.Conclusion MP-DNA testing has important value to the pediatric respiratory infection diagnosis,detection of multiple projects has important significance to preventing illness aggravating.