The effect of ulinastatin on CD4+ CD25+ Foxp3+ regulatory T cells in peripheral blood of patients with acute pancreatitis / 中华普通外科杂志

ABSTRACT

Objective To investigate the effect of ulinastatin (UTI) on peripheral CD4 + CD25 + Foxp3 + Treg cells in patients with acute pancreatitis (AP).Methods Between October 2014 to August 2016,127 patients with AP including three types of severity (MAP,MSAP,SAP) admitted to the Affiliated Union Hospital of Fujian Medical University consecutively were divided into two groups (the UTI group and control group).UTI was added to patients in UTI group.Treg cells were measured by flow cytometry,and the levels of IL-10 were detected by enzyme-linked immunosorbent assay (ELISA).Results On the third and seventh day,the percentage of Treg in UTI group (MAP2.72％ ±0.76％,2.26％ ±0.61％;MSAP3.04％ ±0.74％,4.12％ ± 1.10％;SAP4.41％ ±0.86％,5.38％ ± 1.13％) were higher than that of control group (MAP2.08％ ±0.50％,1.83％ ±0.67％;MSAP2.24％ ±0.89％,3.25％ ±0.70％;SAP3.35％ ±0.85％,4.56％ ±0.73％),all P ＜0.05.For patients with MAP,the levels of IL-10 in UTI group (48.85 ± 15.46) were higher than control group (37.43 ± 13.36) only on the third day.For patients with MSAP and SAP,the levels of IL-10 in UTI groups (MSAP32.95 ± 10.72,59.79 ± 20.09;SAP22.48 ±3.67,30.91 ±8.74) were higher than control groups (MSAP25.15 ± 10.05,36.66 ± 16.80;SAP18.73 ±2.69,23.72 ±5.73) both on the third and seventh day (P ＜0.05).The effective ratio of the treatment in UTI groups were higher than control groups (92.2％ vs.79.4％).Conclusions Ulinastatin regulates the immune function and alleviates inflammatory response during AP by inducing the expansion of Treg cells.