Clinical significance of detection of HE4, OPN and uPA in the diagnosis of endometrial carcinoma / 国际肿瘤学杂志

ABSTRACT

Objective To investigate the expressions and relationships of human epididymal secretory protein 4 (HE4),osteopontin (OPN) and urokinase-type plasminogen activator (uPA) in endometrial carcinoma (EC) and analyze their clinical significance of detection and diagnosis in EC patients.Methods The expressions of HE4,OPN and uPA proteins in 30 cases of normal endometrium,50 cases of precancerous lesion endometrium and 171 cases of EC were detected by immunohistochemistry SP method.The relationships between HE4,OPN,uPA and clinicopathological features of EC,and the correlation of them were analyzed.Results The positive expression rates of HE4,OPN and uPA in the normal endometrium group,precancerous lesion endometrium group and EC group were 13.3％,32.0％,70.8％;10.0％,24.0％,63.7％;6.7％,22.0％,51.5％.The differences were statistically significant (x2 =46.36,P ＜ 0.05;x2 =44.14,P ＜ 0.05;x2 =28.10,P ＜ 0.05).The expressions of HE4,OPN and uPA were correlated withpathological stage (x2 =4.644,P =0.031;x2 =5.780,P =0.016;x2 =28.920,P =0.000),histological grade (x2 =4.888,P =0.027;x2 =7.885,P =0.005;x2 =4.564,P =0.033),myometrial invasion depth (x2 =5.099,P =0.024;x2=5.139 P=0.023;=12.297,P=0.000) and lymph node metastasis (x2 =5.421,P=0.020;x24.093,P =0.043;x2 =5.362,P =0.021).In addition,the expression of HE4 was correlated with the histological type (x2 =4.437,P =0.035).HE4 expression was positively correlated with uPA expression (r =0.341,P =0.007).OPN expression was positively correlated with uPA expression (r =0.360,P =0.002).HE4 was positively correlated with OPN expression (r =0.454,P =0.000).Conclusion The expression rates of HE4,OPN and uPA in the EC group were higher than that in normal endometrium and precancerous lesions.They have obvious positive synergistic effect in the occurrence and development of EC.HE4,OPN and uPA are the effective indicators for clinical diagnosis of EC.