Propofol intervention on mesenchymal stem cell transplantation on brain injury in rats between Human amniotic / 中国生化药物杂志

ABSTRACT

Objective Human amniotic mesenchymal stem cells (AM-MSCs) transplantation and to investigate the effect of propofol treatment, recovery of brain injury in rats. Methods 80 SD rats, male and female, weight 300-350 g. Human amniotic mesenchymal stem cells cultured in vitro and recovery (CM-Dil) before transplantation standby. The fluid percussion brain injury device, give 2.5-3.0 ATM hydraulic impact, severe fluid percussion brain injury model was made. After injury 6h give corresponding treatment which were randomly divided into 4 groups: injury group (medium injection group), AM-MSCs transplantation group and propofol group, AM-MSCs + propofol group. The number of positive cells in each group was observed under the fluorescence microscope using CM-Dil markers. 4 weeks after transplantation, 6 rats were randomly executed, and RT-PCR and Blot Western were used to detect the changes of GAP-43, AQP4 gene expression and protein synthesis in brain tissues. After transplantation, 24 h, 3 days and 1, 2, 3, 4 weeks for animal neurological deficit score, in 4 weeks after transplantation of Morris water maze (Morris Water Maze, MWM) test. They were killed after four weeks by immunohistochemistry, HE staining. Results Number of CM-DIL positive cell was the most in AM-MSCs + propofol group, which was in propofol group and AM-MSCs transplantation group were lower than that in the AM-MSCs + propofol group, which in injury group was the least, and the differences among the groups had statistically significant (P<0.05). 4 weeks after transplantation, AQP4 and its mRNA expression of brain damage surrounding tissue in injury group were higher than those in AM-MSCs transplantation group and propofol group; which in AM-MSCs transplantation group and propofol group was higher than those in AM-MSCs + propofol group (P< 0.05); GAP-43 protein and mRNA expression in injury group were lower than those in AM-MSCs transplantation group and propofol group, which in AM-MSCs transplantation group and propofol group were lower than those in AM-MSCs + propofol group (P<0.05). 1 weeks after transplantation, rat nerve dysfunction score in AM-MSCs + propofol group was lower than that in propofol group and AM-MSCs transplantation group; which in propofol group and AM-MSCs transplantation group was lower than that in the control group (P<0.05). The times of crossing platform in AM-MSCs + propofol group was higher than that in propofol group and AM-MSCs transplantation group, significantly higher than that in the injury group (P<0.01). The pathological section was observed 4 weeks after injury, there was no axonal passage in the injury group. In propofol group and AM-MSCs transplantation group, a few axonal like structures were observed, more axonal like structures were observed in AM-MSCs + propofol group. Conclusion Propofol combined with human amniotic mesenchymal stem cells transplantation can significantly improve the neurological function of rats with brain injury.