DNA-pool high-throughput whole genome resequencing for exploring essential hypertension single nucleotide polymorphism mutation / 国际检验医学杂志
International Journal of Laboratory Medicine
; (12): 1172-1175, 2017.
Article
de Zh
| WPRIM
| ID: wpr-615928
Bibliothèque responsable:
WPRO
ABSTRACT
Objective To use the DNA-pool technology to sequence patients with essential hypertension(EH) for exploring the single nucleotide polymorphism(SNP) mutation situation in Chinese patients with EH.Methods One hundred EH outpatients in the Shenzhen Sun Yat-sen Cardiovascular Hospital from March to June 2014 were continuously collected.The genomic DNA was performed the fragmentation process to 400-800 bp for conducting the database creation and sequencing.The sequencing results were compared with hg19 in the human gene bank(National Center of Biotechnology Information).Results A total of 120.8 Gb original sequence data were generated.The sequencing depth was 36.13 times,the coverage rate reached 99.88%.A total of 4 305 668 SNP loci were detected by the bioinformatic analysis,in which the C:G→T:A motation types were miximal,reaching 12 314 variation sites.Conclusion This study verifies that the data obtained by using the DNA-pool whole genome resequencing method replenishes the Chinese gene database of EH and provides some help for EH gene reasearch in the future.
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WPRIM
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Zh
Texte intégral:
International Journal of Laboratory Medicine
Année:
2017
Type:
Article