Simultaneous determination of eight constituents in Jinsang Liyan Capsules by HPLC / 中成药

ABSTRACT

AIM To develop an HPLC method for the simultaneous content determination of honokiol,magnolol,dehydrotumulosic acid,tumulosic acid,polyporenic acid C,3-epidehydrotumulosic acid,dehydropachymic acid and pachymic acid in Jinsang Liyan Capsules (Magnoliae officinalis Cortex,Poria,Aurantii Fructus Immaturus,etc.).METHODS The analysis of methanol extract of this drug was carried out on a 35 ℃ thermostatic Kromasil C18 column (4.6 mm × 250 mm,5.0 μm),with the mobile phase comprising of acetonitrile-0.05％ phosphoric acid flowing at 0.9 mL/min in a gradient elution manner,and the detection wavelengths were set at 294,241 and 210 nm.RESULTS Eight constituents showed good linear relationships within their own ranges (r ＞ 0.999 0),whose average recoveries were more than 96.0％ with the RSDs of less than 2.0％.CONCLUSION This simple and reproducible method can be used for the quality control of Jinsang Liyan Capsules.