Effect of FAT10 Expression on the Radiation Sensitivity of Human Cervical Carcinoma SiHa Cells in Nude Mice / 医学研究杂志

ABSTRACT

Objective After the detection of FAT10 protein in human cervical cancer tissue behind clinical pathological examination,investigate the effect of up regulation and down regulating the expression of FAT10 gene on the radiation sensitivity of cervical cancer cell line SiHa cells in nude mice.Methods The expression levels of FAT10 mRNA in 30 cases cervical cancer and adjacent tissues were detected by RT-PCR,FAT10 siRNA and recombinant expression vector pcDNA3.1-FAT10 were respectively transfected into cervical cancer SiHa cells by electroporation,and stable transfection cell lines was obtained by G418 screening.After transfection 48h,the expression levels of FAT10 mRNA and protein were respectively detected by fluorescence quantitative RT-PCR and Western blot.Cell proliferation was detected with CCK-8.The effect of FAT10 expression on the radiation sensitivity of cervical cancer cell SiHa line was observed by the clonogenic experiment.The cell apoptosis was detected by TUNEL.The growth of cervical cancer cells was detected by Xenograft tumor assay combined with X-ray irradiation.Results The relative expression level of FAT10 protein in human cervical cancer tissues was significantly higher than that in adjacent tissues,and the difference was statistically significant(P ＜ 0.05).The expression of FAT10 gene in cervical cancer SiHa cells was significantly lower in FAT10 down group,and the FAT10 up regulation group was significantly higher.The radiation sensitivity of cervical cancer SiHa cells was increased in the FAT10 down group,and the radiation sensitivity of SiHa cells was decreased in the FAT10 up regulation group (P ＜ 0.05).FAT10 down group of cervical cancer SiHa cell proliferation was inhibited,the cell apoptosis rate was increased (P ＜ 0.05).The proliferation ability of FAT10 cells was significantly enhanced,and the cell apoptosis rate was decreased in the FAT10 up regulation group (P ＜ 0.05).The average volume of transplanted tumor in the FAT10 down group was significantly smaller than that of the control group (P ＜ 0.05);the average volume of the tumor in the FAT10 up regulation group was larger than that of the control group (P ＜ 0.05).The average volume of the tumor was significantly decreased in the FAT10 down group after irradiation of 20Gy gamma ray(P ＜ 0.05),and the average volume of subcutaneous implanted tumor in nude mice was not significantly different (P ＞ 0.05).Conclusion Downregulation of FAT10 expression can inhibit the growth of human cervical cancer SiHa cells in nude mice,enhance the radiation sensitivity of tumor cells,up regulation of FAT10 expression will make tumor radiation resistance.