Study on chiral recognition for the glimepiride and glimepiride-cis-isomer by applying three amino acid (D-Lysine, L-Glutamine and L-Tyrosine) as chiral probes based on electrospray ionization mass spectrometry / 药物评价研究
Drug Evaluation Research
;
(6): 792-796, 2017.
Article
Dans Chinois
| WPRIM
| ID: wpr-619695
ABSTRACT
Objective Chiral recognition for the glimepiride and glimepiride-cis-isomer by applying three amino acid (D-Lysine,L-Glutamine and L-Tyrosine) as chiral probes based on electrospray ionization mass spectrometry (ESI-MS) was achieved.Methods glimepiride/glimepiride-cis-isomer solutions were mixed with three amino acid solutions.The complex was extracted by ESI-MS and then the fragmentation abundance was investigated applying collision induced dissociation (CID) by MS/MS,which is the basis of chiral recognition for glimepiride and glimepiride-cis-isomer.Results Chiral recognition effect was achieved with the recognition rate (R) 1.61,2.92 and 2.17 for D-Lysine,L-Glutamine and L-Tyrosine respectively.Conclusion 3 kinds ofchiral amino acids were used as probes to distinguish between stereoisomers,and rapid identification of glimepiride and glimepiride cis isomer by mass spectrometry come true for the first time.
Texte intégral:
Disponible
Indice:
WPRIM (Pacifique occidental)
langue:
Chinois
Texte intégral:
Drug Evaluation Research
Année:
2017
Type:
Article
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