Using PCR technique to multiply NS4 gene of hepatitis C virus by deep vent DNA polymerase enzyme
Journal of Practical Medicine
;
: 5-6, 2005.
Article
Dans Vietnamien
| WPRIM
| ID: wpr-6234
ABSTRACT
Process of multiply NS4 gene of hepatitis C virus by PCR technique used with cDNA supplied from ARN. Couple of specific preys for NS4 gene and catalytic enzyme was Deep Vent ADN polymerase. Suitable MgSO4 level to multiply NS4 was 4mM. Fasten prey temperature was factor affecting to specificity of product. Result of electrophoresis showed that the most suitable temperature was 55oC and 57OC. In 200 µM dNTPs level, PCN product was sharp and correct to primary predictable size, in 50 or 100 µM dNTPs level, PCR wasn’t viewed, in 150 µM level, PCR was more blear than using 200 µM level. So dNTPs level was 200 µM
Texte intégral:
Disponible
Indice:
WPRIM (Pacifique occidental)
Sujet Principal:
Réaction de polymérisation en chaîne
/
Hepacivirus
/
Enzymes
/
Gènes
Type d'étude:
Étude pronostique
langue:
Vietnamien
Texte intégral:
Journal of Practical Medicine
Année:
2005
Type:
Article
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