An experimental study on the expression of heme oxygenase-2 mRNA in Hirschsprung's disease / 华中科技大学学报(医学)(英德文版)
Journal of Huazhong University of Science and Technology (Medical Sciences)
; (6): 152-4, 2002.
Article
Dans En
| WPRIM
| ID: wpr-634044
Responsable en Bibliothèque :
WPRO
ABSTRACT
In order to investigate the relationship between the expression of heme oxygenase-2 (HO-2) mRNA and the pathogenesis of Hirschsprung's disease (HD), total ribonucleic acid (RNA) was extracted in the aganglionic and ganglionic segments of colon respectively from 15 cases of HD. The single-stranded cDNA of HO-2 was synthesized and further amplified by reverse transcription-polymerase chain reaction (RT-PCR). The expression of HO-2 mRNA was normal in ganglionic segments, but absent in aganglionic segments. It is concluded that the absence of HO-2 mRNA expression may be an important mechanism responsible for HD.
Texte intégral:
1
Indice:
WPRIM
Sujet Principal:
ARN messager
/
Côlon
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Heme oxygenase (decyclizing)
/
Maladie de Hirschsprung
langue:
En
Texte intégral:
Journal of Huazhong University of Science and Technology (Medical Sciences)
Année:
2002
Type:
Article