Applied study on magnetic nanometer beads in preparation of genechip samples / 华中科技大学学报(医学)(英德文版)
Journal of Huazhong University of Science and Technology (Medical Sciences)
;
(6): 25-7, 2004.
Article
Dans Anglais
| WPRIM
| ID: wpr-634078
ABSTRACT
A protocol for enrichment and adsorption of karyocyte from whole blood by using magnetic nanometer beads as solid-phase absorbents was presented. The PCR amplification could be accomplished by using the nanobeads with karyocyte as template directly and the PCR products were applied on an oligonucleotide array to do gene typing. The HLA-A PCR amplification system and a small HLA-A oligonucleotide microarray were applied as the platform and an experiment protocol of separating karyocyte from whole blood using the magnetic nanometer beads (Fe2O3) were set up. The experimental conditions were also discussed. It showed that pH level of PBS eluent, Taq enzyme quantity and fragment length of products could influent the amplification results, and the magnetic nano-beads could succeed in sample preparation in microarray to provide a promising way in automatic detection and lab-on-a-chip.
Texte intégral:
Disponible
Indice:
WPRIM (Pacifique occidental)
Sujet Principal:
Matrices (génétique)
/
Séparation cellulaire
/
Réaction de polymérisation en chaîne
/
Adsorption
/
Séquençage par oligonucléotides en batterie
/
Nanotechnologie
/
Génotype
/
Leucocytes
/
Magnétisme
/
Microchimie
langue:
Anglais
Texte intégral:
Journal of Huazhong University of Science and Technology (Medical Sciences)
Année:
2004
Type:
Article
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