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PDX-1 expression in pancreatic ductal cells after partial pancreatectomy in adult rats / 华中科技大学学报(医学)(英德文版)
Journal of Huazhong University of Science and Technology (Medical Sciences) ; (6): 464-6, 2004.
Article Dans Anglais | WPRIM | ID: wpr-634174
ABSTRACT
To investigate the protein and mRNA expression of pancreas/duodenal homeobox-1 (PDX-1), a transcription factor as a marker for pancreatic stem cells, in pancreatic ductal cells of rats after partial (90%) pancreatectomy and evaluated the significance of the PDX-1 expression. Western blot and Reverse transcriptase-polymerase chain reaction (RT-PCR) were used to detect the expression of PDX-1 protein and mRNA respectively. PDX-1 protein was only faintly detected in pancreatic ductal cells on the day 1 after partial pancreatectomy. On the day 2 and 3 after operation in operation group, a 2-3 fold increased PDX-1 protein was observed, corresponding to the characteristic 42-kD protein in Western blot. There was significant difference between operation group and sham-operation group (P0.05). RT-PCR revealed the PDX-1 mRNA expression showed no significant difference between operation group at various time points and sham-operation group (P> 0.05). These results indicate that there was overexpression of PDX-1 in the cells of pancreatic epithelium during the regeneration of remnant pancreas after partial pancreatectomy in adult rats, suggesting the pancreatic stem cells in pancreatic ductal epithelial cells are involved in the regeneration of remnant pancreas and the expression of PDX-1 in ductal cells was regulated posttranscription.
Sujets)
Texte intégral: Disponible Indice: WPRIM (Pacifique occidental) Sujet Principal: Pancréatectomie / Conduits pancréatiques / ARN messager / Transactivateurs / Rat Sprague-Dawley / Protéines à homéodomaine / Cellules épithéliales langue: Anglais Texte intégral: Journal of Huazhong University of Science and Technology (Medical Sciences) Année: 2004 Type: Article

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Texte intégral: Disponible Indice: WPRIM (Pacifique occidental) Sujet Principal: Pancréatectomie / Conduits pancréatiques / ARN messager / Transactivateurs / Rat Sprague-Dawley / Protéines à homéodomaine / Cellules épithéliales langue: Anglais Texte intégral: Journal of Huazhong University of Science and Technology (Medical Sciences) Année: 2004 Type: Article