Your browser doesn't support javascript.
loading
Relationship between the expression of MTA-1 gene and the metastasis and invasion in human osteosarcoma / 华中科技大学学报(医学)(英德文版)
Journal of Huazhong University of Science and Technology (Medical Sciences) ; (6): 445-7, 2005.
Article Dans Anglais | WPRIM | ID: wpr-634256
ABSTRACT
To compare the expression level of metastasis associated-1 (MTA1) gene in high and low metastatic human osteosarcoma cell lines and examine the relationship of MTA1 expression and the metastasis potentiality of osteosarcoma cells, the expression of MTA1 in MG-63 osteosarcoma cell lines with high and low metastasis potential was detected by semiquantitative TR-PCR. Boyden chamber invasion assay was used to evaluate the invasive capacity in vitro in two osteosarcoma cell lines. The low metastasis MG-63 cells were transfected with MTA1 full-length cDNA expression plasmid by lipofectamine and the changes of MTA1 expression and in vitro invasion potential were examined after the transfection. Our results showed that MG63 cell line with high metastasis potential expressed significantly higher MTA1 than that of MG63 cells with low metastasis as reavealed by RT-PCR. The invasion potential of low metastasis MG63 cell line was increased after MTA1 gene transfection. It is concluded that there may be a relationship between MTA 1 and invasive potentiality of human osteosarcoma cells, and the mechanism of MTA1 in osteosarcoma metastasis and its possible role in associated gene therapy deserve further study.
Sujets)
Texte intégral: Disponible Indice: WPRIM (Pacifique occidental) Sujet Principal: Protéines de répression / Tumeurs osseuses / ARN messager / Cellules cancéreuses en culture / Ostéosarcome / Régulation de l'expression des gènes tumoraux / Histone deacetylases / Invasion tumorale / Métastase tumorale langue: Anglais Texte intégral: Journal of Huazhong University of Science and Technology (Medical Sciences) Année: 2005 Type: Article

Documents relatifs à ce sujet

MEDLINE

...
LILACS

LIS

Texte intégral: Disponible Indice: WPRIM (Pacifique occidental) Sujet Principal: Protéines de répression / Tumeurs osseuses / ARN messager / Cellules cancéreuses en culture / Ostéosarcome / Régulation de l'expression des gènes tumoraux / Histone deacetylases / Invasion tumorale / Métastase tumorale langue: Anglais Texte intégral: Journal of Huazhong University of Science and Technology (Medical Sciences) Année: 2005 Type: Article