beta-catenin/Tcf signaling in squamous differentiation of porcine airway epithelial cells / 华中科技大学学报(医学)(英德文版)
Journal of Huazhong University of Science and Technology (Medical Sciences)
;
(6): 121-4, 2008.
Article
Dans Anglais
| WPRIM
| ID: wpr-634585
ABSTRACT
For a preliminary study of the role of beta-catenin/Tcf signaling in squamous differentiation of airway (tracheobronchial) epithelial cells, a stable mutant of beta-catenin was transfected into primarily cultured porcine airway epithelial cells. Western blotting revealed that exogenous protein was observed in large quantity in cytoplasm and nucleus. When co-transfected with Tcf luciferase reporter plasmids, beta-catenin mutant increased the reporter's transcriptional activities. However, mRNA expression of a squamous differentiation marker, small proline-rich protein (SPRP), was not elevated, as shown by reverse transcription-polymerase chain reaction. These findings suggest that beta-catenin/Tcf signaling may not be directly involved in the squamous differentiation of porcine airway epithelial cells.
Texte intégral:
Disponible
Indice:
WPRIM (Pacifique occidental)
Sujet Principal:
Suidae
/
Trachée
/
Transcription génétique
/
ARN messager
/
Transduction du signal
/
Différenciation cellulaire
/
Noyau de la cellule
/
Cellules cultivées
/
RT-PCR
/
Cytoplasme
langue:
Anglais
Texte intégral:
Journal of Huazhong University of Science and Technology (Medical Sciences)
Année:
2008
Type:
Article
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