High Expression, Purification and Immuno-indentification of Recombinant HIV Surface Antigen gp120 / 中国病毒学
Virologica Sinica
;
(4): 109-113, 2001.
Article
Dans Chinois
| WPRIM
| ID: wpr-635213
ABSTRACT
Different-length fragments of the HIV trans-membrane antigen (gp120) gene were expressed in Escherichia coli, using T7 expression system. SDS-PAGE stained by Coomassic Brilliant Blue showed no expression of full-length gp120 and poor expression of half-length gp120 fragments from the N-terminal, but high expression of 1/3-lgenth gp120 gene fragments from the N-terminal (including V1/V2 epitopes), more than 18% of total bacterial protein. Western blot showed fairly good reactivity to serum from HIV-infected individual. On this basis, we expressed the corresponding tp 120 fragments of the HIV strains epidemic in China. This study laid a solid foundation for exploration of high expression of gp 120 in E.coli and development of HIV serological diagnostic system for Chinese people.
Texte intégral:
Disponible
Indice:
WPRIM (Pacifique occidental)
langue:
Chinois
Texte intégral:
Virologica Sinica
Année:
2001
Type:
Article
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