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Comparative Analysis of Serological and Molecular Methods for the detection of Rice Grassy Stunt Virus / 中国病毒学
Virologica Sinica ; (4): 361-366, 2000.
Article de Zh | WPRIM | ID: wpr-635270
Bibliothèque responsable: WPRO
ABSTRACT
Methods of ELISA, nonradioactive molecular hybridiz ation and RT-PCR were applied in the detection of rice grassy stunt virus (RGSV ). The detection sensitivity of indirect ELISA using antiserum against fusion p rotein GST-NC was 1 mg of infected leaves or 84 ng of purified virus. The metho d of dot hybridization using NC, a DIG-labelled DNA probe was 50 μg diseased l e aves, or 6 ng purified preparations. The detection endpoint of RT-PCR was 10 μg diseased leaves, or 2 ng purified virus preparation. Comparisons of sensitivit y and maneuverability were made among these methods.
Mots clés
Texte intégral: 1 Indice: WPRIM Type d'étude: Diagnostic_studies langue: Zh Texte intégral: Virologica Sinica Année: 2000 Type: Article
Texte intégral: 1 Indice: WPRIM Type d'étude: Diagnostic_studies langue: Zh Texte intégral: Virologica Sinica Année: 2000 Type: Article