Changes of Natural Killer Cells and T Lymphocyte Subpopulations in Neonates with Hypoxic-Ischemic Encephalopathy and Its Correlation Factors / 实用儿科临床杂志

ABSTRACT

Objective To investigate the changes and correlative factors of natural killer(NK)cells and T lymphocyte subpopulations levels in neonates with hypoxic-ischemic encephalopathy(HIE).Methods Peripheral blood samples were collected from 50 neonates with HIE.The percentages of NK cell(CD3~-CD_(16)~+CD_(56)~+) and T lymphocyte subpopulations including CD3~+,CD4~+,CD8~+ and CD4~+/CD8~+ ratio were determined by Flow Cytometr(FCM).Meanwhile,20 healthy neonates were served as controls.Results (Le)-vels of NK cell,CD3~+,CD4~+,CD8~+ and CD4~+/CD8~+ ratio in neonates with HIE were significantly lower than those in control group(all P0.05).Spearman correlation analysis showed that there were negative correlation between the NK cell,CD3~+,CD4~+,CD4~+/CD8~+ ratio and stages of HIE(all P0.05). Linearregression analysis showed that there was a positive correlation between NK cell and gestationalages((P