Animal Model for Sequential Bilateral Reimplantation of the Lung in Dog / 대한흉부외과학회지

ABSTRACT

Sequential bilateral lung transplantation may result in a variety of perioperative and postoperative complications, showing high perioperative morbidity and mortality rates. This research was performed to investigate the hemodynamic changes in adult mongrel dogs after bilateral reimplantation, two methods preferred for avoiding or minimizing graft rejection. The anterior portion of the pulmonary artery and the left atrium proximal to the superior and the inferior pulmonary veins were resected out and then re-anastomosed one hour later to prevent torsion or stenosis of the anastomotic site and the formation of a thrombosis in the left atrium. An everted suture technique was employed for the left atrium; An hour after the division, however, the main bronchus was tightly anastomosed by interrupted sutures of No. 4-0 prolene in a telescope method. A modified E-C solution mixed with PGE1 was infused into the cut portion of the pulmonary artery at the rate of 15 ml/kg/min and at a pressure of 40 cmH2O for a total dosage of 70 ml/kg in order to preserve the transected lung. Topical cooling using wet gauzes soaked with cold E-C solution was performed for one hour to prevent ischemic lung injury. The above procedures are considered to be beneficial for achieving a satisfactory outcome for bilateral lung reimplantation.