Effects of active component of Zhimu on APP and BACE1 in HEK293sw cells / 上海第二医科大学学报
Article
Dans Zh
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| ID: wpr-640741
Responsable en Bibliothèque :
WPRO
ABSTRACT
Objective To investigate the effects of ZMS,the active component of Zhimu,on amyloid precursor protein(APP) and ?-site APP cleaving enzyme 1(BACE1) in HEK293sw cells. Methods HEK293sw cells were pretreated with different concentrations of ZMS in routine culture medium for 24 h,and then serum-free medium with ZMS was used for further culture for 48 h.Cells were examined for expression of APP and ?-secretase BACE1 with Western blotting and RT-PCR,and were then examined for BACE1 activity with fluorescence method. Results 1 ?mol/L and 10 ?mol/L ZMS significantly decreased the expression of BACE1 mRNA and protein,while had no effect on the expression of APP mRNA and protein.It was indicated by fluorescence analysis that 10 ?mol/L ZMS significantly decreased the ?-secretase activity.Conclusion ZMS significantly decreases the activity and expression of ?-secretase BACE1,while has no effect on the expression of APP in HEK293sw cells.
Texte intégral:
1
Indice:
WPRIM
langue:
Zh
Texte intégral:
Journal of Shanghai Jiaotong University(Medical Science)
Année:
2006
Type:
Article