Interactome Analysis Reveals that Heterochromatin Protein 1gamma (HP1gamma) Is Associated with the DNA Damage Response Pathway / Journal of the Korean Cancer Association, 대한암학회지
Cancer Research and Treatment
;
: 322-333, 2016.
Article
Dans Anglais
| WPRIM
| ID: wpr-64173
ABSTRACT
PURPOSE:
Heterochromatin protein 1gamma (HP1gamma) interacts with chromosomes by binding to lysine 9-methylated histone H3 or DNA/RNA. HP1gamma is involved in various biological processes. The purpose of this study is to gain an understanding of how HP1gamma functions in these processes by identifying HP1gamma-binding proteins using mass spectrometry. MATERIALS ANDMETHODS:
We performed affinity purification of HP1gamma-binding proteins using G1/S phase or prometaphase HEK293T cell lysates that transiently express mock or FLAG-HP1gamma. Coomassie staining was performed for HP1gamma-binding complexes, using cell lysates prepared by affinity chromatography FLAG-agarose beads, and the bands were digested and then analyzed using a mass spectrometry.RESULTS:
We identified 99 HP1gamma-binding proteins with diverse cellular functions, including spliceosome, regulation of the actin cytoskeleton, tight junction, pathogenic Escherichia coli infection, mammalian target of rapamycin signaling pathway, nucleotide excision repair, DNA replication, homologous recombination, and mismatch repair.CONCLUSION:
Our results suggested that HP1gamma is functionally active in DNA damage response via protein-protein interaction.
Texte intégral:
Disponible
Indice:
WPRIM (Pacifique occidental)
Sujet Principal:
Spectrométrie de masse
/
Phénomènes biologiques
/
Altération de l'ADN
/
Cytosquelette d'actine
/
ADN
/
Hétérochromatine
/
Histone
/
Chromatographie d'affinité
/
Splicéosomes
/
Jonctions serrées
langue:
Anglais
Texte intégral:
Cancer Research and Treatment
Année:
2016
Type:
Article
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