Characterization of Clostridium difficile Strains Isolated from Patients with C. difficile-associated Disease in Korea
Osong Public Health and Research Perspectives
;
(6): 325-331, 2017.
Article
Dans Anglais
| WPRIM
| ID: wpr-644319
ABSTRACT
OBJECTIVES:
Studies on Clostridium difficile are rare in Korea. We investigated the epidemiological characteristics of C. difficile isolates from patients with C. difficile-associated disease (CDAD) in Korea.METHODS:
Multiplex polymerase chain reaction was performed to detect the presence of tcdA and tcdB toxin genes. Antimicrobial susceptibility test was carried out by the disk-dilution method. C. difficile strains were subtyped by automated repetitive-element palindromic PCR (rep-PCR).RESULTS:
Among patients with CDAD, 73 (25.8%), 32 (11.3%), 32 (11.3%), and 26 (9.2%) suffered from pneumonia, cancer or neoplasm, diabetes, and colitis, respectively. Of all stool samples, 43 samples (15.2%) were positive for C. difficile strains. We observed two expression patterns of toxin genes tcdA+/tcdB+ (86% isolates) and tcdA−/tcdB+ (14% isolates), with all isolates expressing tcdB. Furthermore, some isolates were resistant to clindamycin (65%), ampicillin (56%), and cefazolin (40%), but all were susceptible to vancomycin and metronidazole. The tested samples were classified into diverse clusters using automated rep-PCR.CONCLUSION:
Our findings revealed the characteristics and antibiotic resistance of C. difficile isolates from patients in Korea. The epidemiological data may provide valuable insight into development of treatment strategies for C. difficile infections in Korea.
Texte intégral:
Disponible
Indice:
WPRIM (Pacifique occidental)
Sujet Principal:
Pneumopathie infectieuse
/
Résistance microbienne aux médicaments
/
Clindamycine
/
Vancomycine
/
Céfazoline
/
Réaction de polymérisation en chaîne
/
Clostridioides difficile
/
Clostridium
/
Colite
/
Réaction de polymérisation en chaine multiplex
Limites du sujet:
Humains
Pays comme sujet:
Asie
langue:
Anglais
Texte intégral:
Osong Public Health and Research Perspectives
Année:
2017
Type:
Article
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