Increase of Rhinovirus Replication in Airway Epithelial Cells by Staphylococcal Enterotoxin A and B / 대한이비인후과학회지
Korean Journal of Otolaryngology - Head and Neck Surgery
;
: 1071-1076, 2006.
Article
Dans Coréen
| WPRIM
| ID: wpr-645098
ABSTRACT
BACKGROUND AND OBJECTIVES:
The toxins generated from Staphylococcus aureus, Staphylococcal enterotoxin A (SEA) and B (SEB), are reported to have an important role in the pathogenesis of chronic rhinosinusitis. As a basic step for elucidating the pathophysiologic responses of the nasal mucosa of chronic rhinosinusitis associated with rhinovirus infection, this study investigated the effect of SEA and SEB on rhinovirus infection in A549 cells. MATERIALS ANDMETHOD:
The effect of SEA and SEB on the rhinovirus-induced changes in intercellular adhesion molecule-1 (ICAM-1) expression was assessed by flow cytometry. The effect of staphylococcal toxins on the rhinovirus-induced cytokine secretion was measured by ELISA. The effect of the replication of rhinovirus in the cells was examined by viral culture with subsequent determination of viral titer.RESULTS:
ICAM-1 expression was increased in the rhinovirus infection group. Cytokine secretion was also increased in the rhinovirus infection group. But there was no additional increase due to staphylococcal toxins regarding the ICAM-1 expression and cytokine secretions. Staphylococcal toxins increased viral titer in proportion to toxin concentrations.CONCLUSION:
SEA and SEB increased rhinoviral replication in airway epithelial cells. This result shows that airway epithelial cells with chronic rhinosinusitis are more favorable environments for rhinovirus infection.
Texte intégral:
Disponible
Indice:
WPRIM (Pacifique occidental)
Sujet Principal:
Rhinovirus
/
Staphylococcus aureus
/
Test ELISA
/
Molécule-1 d'adhérence intercellulaire
/
Entérotoxines
/
Cellules épithéliales
/
Cytométrie en flux
/
Muqueuse nasale
langue:
Coréen
Texte intégral:
Korean Journal of Otolaryngology - Head and Neck Surgery
Année:
2006
Type:
Article
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