Functional Studies of Acyl-CoA Synthetase 4 in the Rat Liver
The Korean Journal of Nutrition
;
: 376-381, 2003.
Article
Dans Coréen
| WPRIM
| ID: wpr-647903
ABSTRACT
Acyl-CoA synthetase 4 (ACS4) is an arachidonate-preferring enzyme abundant in steroidogenic tissues. We examined ACS4 in rat liver, which contains a variety of pathways that use acyl-CoAs, in order to determine subcellular locations. We demonstrate that ACS4 protein was present most abundantly in the mitochondria and to a much lesser extent in the peroxisomes and microsomes. To determined the dietary effects on the level of ACS4 mRNA, northern blotting was carried out using total RNA from the livers of adult male rats fed various diets. Fasting, high fat diet, and fat-free high sucrose diet increased the hepatic level of ACS4 mRNA approximately 2-fold. Furthermore, the levels of ACS4 mRNA were induced by DEHP[Di- (2-ethylhexyl) phthalate]. These data suggest that ACS4 expression in the liver is regulated with a variety of pathways, including beta-oxidation, hormone, and insulin.
Texte intégral:
Disponible
Indice:
WPRIM (Pacifique occidental)
Sujet Principal:
Saccharose
/
ARN
/
ARN messager
/
Technique de Western
/
Technique de Northern
/
Jeûne
/
Acide arachidonique
/
Péroxysomes
/
Régime alimentaire
/
Alimentation riche en graisse
Limites du sujet:
Animaux
/
Humains
/
Mâle
langue:
Coréen
Texte intégral:
The Korean Journal of Nutrition
Année:
2003
Type:
Article
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