Intraotic drug delivery following gentamicin Infusion using the an osmotic pump in chinchilla / 대한이비인후과학회지
Korean Journal of Otolaryngology - Head and Neck Surgery
;
: 458-465, 2001.
Article
Dans Coréen
| WPRIM
| ID: wpr-648563
ABSTRACT
BACKGROUNDS AND OBJECTIVES:
We developed a method of intraotic treatment using gentamicin (GM) in chinchillas. MATERIALS ANDMETHOD:
An osmotic pump, connected near the round window by a Teflon tube (0.13mm ID X 0.18mm OD) through an opening in the scala tympani, continuously perfused 12microliter of GM per day for a week. In the three experimental groups (n=12), each animal was treated with a total dose of 0.1mg of GM for a week. Animals in the control group (n=4) were immediately sacrificed after perfusion with saline for a week.RESULTS:
In the control group, there was no damage in any of the hair cells. In the treatment group, the hair cells were more severely damaged at 4 weeks than at 1 week. The outer hair cells were more sensitive to GM than the inner hair cells. Among the outer hair cells, the first rows were damaged first. The basal part of cochlea was damaged earlier than the apical. The ototoxic effect continued even after perfusion had ceased.CONCLUSIONS:
This pump system limits drug distribution to the target tissue, minimizing systemic side effects while permitting precise control of delivery rate, volume, concentration and duration.
Texte intégral:
Disponible
Indice:
WPRIM (Pacifique occidental)
Sujet Principal:
Perfusion
/
Polytétrafluoroéthylène
/
Rampe tympanique
/
Gentamicine
/
Microscopie électronique à balayage
/
Chinchilla
/
Cochlée
/
Poils
Limites du sujet:
Animaux
langue:
Coréen
Texte intégral:
Korean Journal of Otolaryngology - Head and Neck Surgery
Année:
2001
Type:
Article
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