Characteristics of protein from red crab (Chionoecetes japonicus) shell by commercial proteases
The Korean Journal of Nutrition
; : 429-436, 2012.
Article
Dans Ko
| WPRIM
| ID: wpr-651206
Responsable en Bibliothèque :
WPRO
ABSTRACT
This study was performed to examine the characteristics of protein of red crab (Chionoecetes japonicus) shell powder hydrolyzed by commercial proteases. Red crab shell was digested by commercial proteases, such as Protamex (P), Neutrase (N), Flavourzyme (F), Alcalase (A), Protease M (PM) and Protease A (PA). Protein yield analyzed by Biuret assay, absorbance at 280 nm and brix revealed that PA was the enzyme having the highest proteolytic activity. SDS PAGE showed that molecular weight of proteins produced by protease treatments was various and below 150 kDa. Combinational treatment of proteases (PA + P, PA + PM, PA + F, PA + A) was tried whether these increase protein hydrolysis from red crab shell powder compared to a PA single treatment. Soluble protein content was similar, but amino acid concentration by combinational treatments was higher than PA single treatment [PA + P 247.4 mg/g > PA + F (206.4 mg/g) > PA + A (133.4 mg/g) > PA + PM (59.1 mg/g) > PA (54.9 mg/g)]. Amino acid composition by combinational treatments was slightly different. Most abundant essential amino acids were phenylalanine, glycine, alanine, and leucine, whereas tyrosine and cystine were not detected.
Texte intégral:
1
Indice:
WPRIM
Sujet Principal:
Peptide hydrolases
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Endopeptidases
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Phénylalanine
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Tyrosine
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Biuret
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Metalloendopeptidases
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Subtilisines
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Protéines
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Cystine
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Alanine
langue:
Ko
Texte intégral:
The Korean Journal of Nutrition
Année:
2012
Type:
Article