Anticancer Effect and Apoptosis Mechanism of Photochemotherapy Using Photogem and 632 nm Diode Laser on Human Squamous Cell Carcinoma Cell Line / 대한이비인후과학회지

ABSTRACT

BACKGROUND AND OBJECTIVES: This study was conducted to investigate the anticancer effect of photochemotherapy in vitro using a photosensitizing agent (Photogem) and a laser therapy (632 nm diode laser). MATERIALS AND METHOD: Human squamous cell carcinoma cell (SNU-1041) was treated to laser therapy for various irradiation times, laser powers, and interval times. The treated cell was analyzed by MTT assay, DAPI staining to see apoptosis. RESULTS: The viability of cells was decreased with the increasing of the laser irradiation time and the laser power. No significant difference in cell viability was noted by various interval time. Increasing apoptosis was observed by increasing concentration of Photogem and increasing lasering time by DAPI staining. CONCLUSION: This study demonstrated anticancer effect of photochemotherapy using Photogem and 632 nm diode laser. Apoptosis was observed in the process of cancer cell death.