Effective DNA extraction method to improve detection of Mycobacterium avium subsp. paratuberculosis in bovine feces / 대한수의학회지
Korean Journal of Veterinary Research
;
: 55-57, 2014.
Article
Dans Anglais
| WPRIM
| ID: wpr-65249
ABSTRACT
Paratuberculosis caused by Mycobacterium avium subsp. paratuberculosis (MAP) has extended latent periods of infection. Due to this property, difficulties in the detection of fecal shedder have been raised. A newly designed method for DNA extraction from fecal specimens, mGITC/SC was evaluated in terms of diagnostic efficiency. The detection limit of IS900 real-time PCR was about 50 MAP (1.5 cfu) in 250 mg of feces (6 cfu per g). Also, this DNA extraction method was faster and cheaper than that using commercial kit or other methods. Consequently, the mGITC/SC is an economical DNA extraction method that could be a useful tool for detecting MAP from fecal specimens.
Texte intégral:
Disponible
Indice:
WPRIM (Pacifique occidental)
Sujet Principal:
Paratuberculose
/
ADN
/
Fèces
/
Limite de détection
/
Réaction de polymérisation en chaine en temps réel
/
Mycobacterium
/
Mycobacterium avium
Type d'étude:
Etude diagnostique
Limites du sujet:
Animaux
langue:
Anglais
Texte intégral:
Korean Journal of Veterinary Research
Année:
2014
Type:
Article
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