Effect of beta-Ig H3 on Chondrogenesis by Human Bone Marrow Mesenchymal Stem Cells / 대한정형외과학회잡지
The Journal of the Korean Orthopaedic Association
;
: 373-379, 2004.
Article
Dans Coréen
| WPRIM
| ID: wpr-653334
ABSTRACT
PURPOSE:
This study was undertaken to compare the activity of beta-Ig H3 (BigH3) and transforming growth factor (TGF)-beta on chondrogenesis by mesenchymal stem cells (MSCs). MATERIALS ANDMETHODS:
MSCs in human bone marrow aspirated from 20 healthy donors were isolated by density gradient Ficoll-hypaque separation and expanded in culture. MSC-alginate beads were prepared and incubated for 28 days in the presence of 0.5 or 10 ng/mL of TGF-beta 1, TGF-beta 1+TGF-beta 3 or BigH3. Cellular viability, total collagen and glycosaminoglycan (GAG) contents were measured and compared. SPSS version 9.0 was used for the statistical analysis.RESULTS:
TGF-beta 3 significantly enhanced cell viability in beads by day 21 (p=0.029). No significant differences were found in terms of cell viability (p=0.197) or in total GAG content (p=0.253) between 10 ng/mL of TGF-beta 1+3 and 10 ng/mL of BigH3. Total collagen content was higher in the BigH3 added group on day 21 (p=0.041).CONCLUSION:
The replacement of BigH3 instead of TGF-beta produced appropriate external signals indicating the chondrogenic differentiation of human bone marrow MSCs.
Texte intégral:
Disponible
Indice:
WPRIM (Pacifique occidental)
Sujet Principal:
Donneurs de tissus
/
Moelle osseuse
/
Facteurs de croissance transformants
/
Survie cellulaire
/
Facteur de croissance transformant bêta
/
Collagène
/
Chondrogenèse
/
Cellules souches mésenchymateuses
Limites du sujet:
Humains
langue:
Coréen
Texte intégral:
The Journal of the Korean Orthopaedic Association
Année:
2004
Type:
Article
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