Comparison and evaluation different methods for detecting metallo-β-lactamase / 国际检验医学杂志
International Journal of Laboratory Medicine
; (12): 2523-2525, 2017.
Article
Dans Zh
| WPRIM
| ID: wpr-659386
Responsable en Bibliothèque :
WPRO
ABSTRACT
Objective To evaluate the applicationof the double-disk synergy test(DDST) and combined disk test(CDT) in clini cal metal enzyme phenotype deteetion.To evaluate the value of multiplex PCR in detecting the metallo-β-1actamase in clinical.Methods 56 strains of metallo-β-1actamase-positive strains were identified [NDM-1(n=9),VIM(n=32),IMP(n=15)]for appraise the two methods.By optimizing the design of PCR primers,3 pairs of primers were designed and detected (IMP,VIM,NDM-1) in one tube for evaluate the method.Results The DDST was 80.36%(45/56),and 100.00%(56/56) in the CDT.The accuracy of multiplex PCR was 100.00 % (56/56),and the size of the amplified fragment was used to distinguish three types of metallo-β-lactamase.Conclusion The CDT is more suitable for clinical application than DDST.Multiplex PCR has the characteristics of simple,rapid and accurate in detection of metallo-β-1actamase.It is suitable for daily use of clinical microbiological laboratory,which will help the clinical timely and effective administration.
Texte intégral:
1
Indice:
WPRIM
Type d'étude:
Prognostic_studies
langue:
Zh
Texte intégral:
International Journal of Laboratory Medicine
Année:
2017
Type:
Article