Development of Neutralization Assay using Murine Leukemia Virus (MuLV) Pseudotyped with Japanese encephalitis Virus (JEV) env Gene
Journal of Bacteriology and Virology
;
: 23-30, 2007.
Article
Dans Coréen
| WPRIM
| ID: wpr-66408
ABSTRACT
The envelope (E) glycoprotein of JEV is the major antigen to elicit neutralizing antibody (NAb) against JEV infection. In order to develop a rapid and safe neutralization assay system for evaluation of the JEV vaccine strains, we constructed JEV-pseudotyped viruses with JEV env genes (Nakayama-NIH, Beijing-1). The titers of JEV-pseudotyped viruses with NK and BJ strains were 4.0x10(4) IFU/ml and 1.3x10(5) IFU/ml in Vero cell cultures, respectively. We have analyzed the neutralization activity of immunized mouse sera with JEV-NK and JEV-BJ pseudotyped viruses. The neutralizing antibody titers of NK and BJ (50% reduction of virus) were about 110,000 at each immunized sera. Compared with conventional plaque reduction neutralization test (PRNT), the method using JEV-pseudotyped virus has desirable advantages such as more rapid, easier, and non-biohazardous. This neutralization assay system might be useful to evaluate NAb activity against JEV vaccine strains or vaccine candidates.
Texte intégral:
Disponible
Indice:
WPRIM (Pacifique occidental)
Sujet Principal:
Cellules Vero
/
Tests de neutralisation
/
Glycoprotéines
/
Gènes env
/
Encéphalite japonaise
/
Virus de la leucémie murine
/
Asiatiques
/
Virus de l'encéphalite japonaise (espèce)
/
Anticorps neutralisants
Limites du sujet:
Animaux
/
Humains
langue:
Coréen
Texte intégral:
Journal of Bacteriology and Virology
Année:
2007
Type:
Article
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