Screening and identification of prognosis-related miRNA in hepatocellular carcinoma by the TCGA database / 临床检验杂志

ABSTRACT

Objective MicroRNAs(miRNAs) play a critical role in the prognosis of liver cancer,especially in hepatocellular carcinoma (HCC).The aim of this study was to find the new prognosis-related miRNAs in HCC through data mining of The Cancer Genome Atlas(TC-GA).Methods The miRNA expression data with clinicopathological characteristics and prognosis of liver cancer patients were obtained from the TCGA data portal and then data analysis,integration and standardization were performed by R package.Moreover,univariate Cox model and high-dimensional analytical model BhGLM(Bayesian hierarchical generalized linear model) were used to analyze the miRNA expression profiles data.Results A total of 63 and 77 prognosis-related miRNAs were screened using Cox and BhGLM model respectively,and 18 miRNAs were screened by co-analysis of Cox and BhGLM.Among them 4 prognosis-related miRNAs such as miR-188,miR-576,miR-887 and miR-91 have never been reported in HCC.Conclusion On the basis of these data,co-analysis of Cox and BhGLM could be an effective method which screen and validate miRNAs as the new clinical prognostic and diagnostic biomarkers in HCC.