Preliminary study of flavonoids affecting cAMP level in cannabinoid receptor overexpressed cells / 上海交通大学学报(医学版)
Journal of Shanghai Jiaotong University(Medical Science)
; (12): 1623-1629, 2017.
Article
de Zh
| WPRIM
| ID: wpr-665383
Bibliothèque responsable:
WPRO
ABSTRACT
Objective·To evaluate the effects of flavonoids on cyclic adenosine monophosphate (cAMP) level in cannabinoid receptor (CBR) overexpressed cells. Methods·Eukaryotic expression vectors GV144-CB1R and GV144-CB2R were constructed, and transfected into HEK-293 cells to establish CBR overexpressed cell lines. Several flavonoid compounds were studied by measuring the change of cAMP level in CBR-overpressed HEK-293 cell lines. Results·The CBR overexpressed cell lines were constructed successfully. cAMP level in CBR-overexpressed HEK-293 cells decreased after incubation with flavonoid compounds. And these effects were selective for CB2R overexpressed cells. Conclusion·All flavonoids studied exhibited decreasing effect on cAMP level in CBR-overexpressed cells at μmol/L level.
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Indice:
WPRIM
langue:
Zh
Texte intégral:
Journal of Shanghai Jiaotong University(Medical Science)
Année:
2017
Type:
Article