Simultaneous determination of ten constituents in Dengzhan Xixin Injection by HPLC / 中成药

ABSTRACT

AIM To establish an HPLC method for the simultaneous content determination of 5-caffeoylquinic acid,chlorogenic acid,4-caffeoylquinic acid,caffeic acid,1,3-dicaffeoylquinic acid,scutellarin,isochlorogenic acid B,3,5-dicaffeoylquinic acid,apigenin-7-O-glucronide and 4,5-dicaffeoylquinic acid in Dengzhan Xixin Injection [Erigeron breviscapus (Vaniot.) Hand.-Mazz].METHODS The analysis of aqueous extract of this drug was performed on a 30 ℃ thermostatic Inertsil(R) ODS-3 column (250 mm × 4.6 mm,5 μm),with the mobile phase comprising of 0.4％ phosphoric acid-acetonitrile flowing at 0.9 mL/min in a gradient elution manner,and the detection wavelength was set at 327 nm.RESULTS Ten constituents showed good linear relationships within their own ranges (r≥0.999 0),whose average recoveries were 94.11％-100.64％ with the RSDs of 1.12％-2.77％.CONCLUSION This simple,accurate and reproducible method can be used for the quality control of Dengzhan Xixin Injection.