Effects of milrinone on LPS-induced inflammation of human coronary artery endothelial cells / 中国免疫学杂志

ABSTRACT

Objective:To observe the effects of milrinone on LPS-induced inflammation and NF-κB activation of human coronary artery endothelial cells (HCAEC).Methods:HCAEC were cultured in vitro,and were divided into normal group,LPS group (1 μg/ml),milrinone 50 μmol/L group and milrinone 200 μmol/L group,the rear two groups were co-incubated with LPS for 24 h. qRT-PCR and ELISA was applied to detect IL-6,IL-8,TNF-α mRNA and protein,respectively.Western blot and immunofluorescence assay was used to observe the activation and translocation of NF-κB,respectively.Results: Compared with LPS group,the IL-6,IL-8, TNF-α mRNA and protein;the activation and translocation of NF-κB significantly inhibited after incubation with milrinone (P<0.05) in a dose dependent manner.Conclusion:Milrinone inhibited the release of inflammative cytokines induced by LPS of HCAEC in vitro, partly by inactivation of NF-κB.