Fabrication of trachea cartilage ring by tissue engineering / 实用口腔医学杂志

ABSTRACT

砄bjective To study the feasibility of fabrication of trachea cartilage ring by tissue engineering.Methods: PGA non woven mesh was put into the mold of trachea cartilage ring and enforced with polylactic acid. Rabbit chondrocytes were harvested by collagenase type Ⅱdigestion of ear cartilage and seeded into PGA scaffold in the density of 5?10 7/ml.The cell polymer complexes were incubated in vitro for 1 week and then implanted subcutaniously into the back of nude mice. The formation of trachea cartilage ring was observed by gross inspection and histological examination 2 months after implantation. Results: New cartilage tissue in the shape of trachea ring was found 2 months after implantation. The specimens showed the appearance of glisteringly white with good flexibility. Histological examination demonstrated that newly restored tissue was constituted of cartilage. Conclusion: It may be an efficient method to fabricate trachea cartilage ring by seeding chondrocytes in PGA scaffold.