Sequencing VP4, VP7, NSP1, NSP4 genes of human rotavirus strain G1P8
Journal of Preventive Medicine
;
: 27-32, 2007.
Article
Dans Vietnamien
| WPRIM
| ID: wpr-673
ABSTRACT
Background:
Rotavirus is the main cause of acute viral gastroenteritis in children under 5 years old. The virus leads to over 600000 children deaths a year in the world, 80% of which occur in the developing countries. In Viet Nam, 50%-70% the children\u2019s hospitalizations for acute diarrhea were resulted from rotavirus infection.Objective:
To sequence nucleotides and amino acids of VP4, VP7, NSP1, and NSP4 genes of 5 passages of human rotavirus strain G1P8. Materials andmethod:
A study was conducted in rotavirus sample of 5 passages of human rotavirus strain G1P8 B17A3; B17.3; B17.3 pp32vero15; B17.3 pp36TKP2; B17.3 pp43.7vero in Centre for Disease Control and Prevention, Atlanta, United State.Methods:
using NucliSen Kit for detection of ARN; RT-PCR; sequencing genes by ABI 3100 machine. Results andConclusion:
Sequencing nucleotides and amino acids of VP4, VP7, NSP1, and NSP4 genes of 5 passages of human rotavirus strain G1P8 showed that the number of nucleotide mutations ofVP4, VP7, NSP4 genes occurring among the passages were 3 (at nucleotit 175, 419, 790), 1 (at nucleotit 644), 3 (at nucleotit 134, 254, 482), respectively. All these mutations resulted in changes in amino acid composition. No mutation was found in NSP1 gene.
Texte intégral:
Disponible
Indice:
WPRIM (Pacifique occidental)
Sujet Principal:
Rotavirus
/
Gènes
/
Nucléotides
langue:
Vietnamien
Texte intégral:
Journal of Preventive Medicine
Année:
2007
Type:
Article
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