Effect of metformin on human choriocarcinoma cell apoptosis / 国际检验医学杂志

ABSTRACT

Objective To investigate the effects of metformin on apoptosis of human choriocarcinoma cell and its possible action mechanism .Methods The human choriocarcinoma cell line JEG‐3 was selected and divided into the control group and metformin groups(final concentrations of 5 ,10 ,20 ,40 mmol/L) .Flow cytometry and immunofluorescence assay were adopted to detect cell ap‐optosis at 48 h after treatment .The mRNA and protein change trend of Caspase‐3 ,Bcl‐2 and Bax were measured by Real‐time PCR and Western Bolt .Results Compared with the control group ,the early and late apoptosis rate of JEG‐3 cells in the metefomin groups were remarkably increased ,meanwhile mRNA and protein expression levels of Caspase‐3 and Bax were significantly in‐creased ,but Bcl‐2 mRNA and protein expression were significantly decreased .Conclusion Metformin induces the JEG‐3 cells to generate apoptosis by the Caspase‐3 and Bcl‐2/Bax pathways .