Antiepileptic drug-induced overexpression of P-glycoprotein multidrug resistance gene in astrocytes in vitro / 中华神经科杂志

ABSTRACT

Objective To study the overexpression of antiepileptic drug (AED) induced P glycoprotein multidrug resistance gene in astrocytes in vitro Methods Postnatal Wistar rats (within 24 h) astrocyte cell culture was performed. Different concentrations of phenytoin (PHT),phenobarbitone (PB),carbamazepine (CBZ),valproate (VPA) were added to the culture media for 10,20 and 30 days The overexpression of P glycoprotein (Pgp) multidrug resistance gene was investigated with the immunocytochemistry method Results The Pgp staining of normal astrocytes was less than 5% at each time point The Pgp positive staining was found in 20 mg/L PB,40 mg/L PB,20 mg/L PHT at the 30 day There were overexpressions at the 20 and 30 day, excluding CBZ at 20 day in the higher concertration of the four AED Conclusion AED may induce the overexpression of Pgp,which is correlated with the dose and time